Effect of Piezo1 Overexpression on Peritumoral Brain Edema in Glioblastomas

A, Relative Piezo1 levels in clinical samples of GBM and paired adjacent normal tissues in a cohort of 64 patients with GBMs with WHO grade IV. B, Piezo1 expression was detected in several representative paired GBM samples. N indicates adjacent normal tissues; T, tumor tissues.

A–I, MR imaging (T2-weighted scan and T1-enhanced scan) and Piezo1 immunohistochemical staining of tumors with different grades of brain edema (mild edema, moderate edema, and severe edema). J, Piezo1 expression levels in the mild edema group (n = 9), moderate edema group (n = 16), and severe edema group (n = 39) were assessed by IHC. K, The EI was compared between the high Piezo1 expression group (n = 44) and the low Piezo1 expression group (n = 20). L, Scatterplot of Piezo1 expression and its corresponding EI based on linear regression analysis (R² = 0.47, P < .001). The asterisk indicates P < .05, 4 asterisks, P < .001 using a 2-tailed Student t test.

A and B, Scatterplot of Piezo1 expression and its corresponding EI based on linear regression analysis in the high- and low-expression subgroups of Ki-67 (R² = 0.33, P < .001; R² = 0.75, P < .001; respectively). C and D, In the subgroup analysis of Ki-67, the EI was compared between the high-Piezo1 and low-Piezo1 expression groups. The asterisk indicates P < .05; 4 asterisks, P < .001 using a 2-tailed Student t test.