Apoptosis in Leukoaraiosis

Luxol fast blue–stained section from the brain slice imaged in figure 1. The outlined area corresponds to the area of leukoaraiosis in the MR image in figure 1. Reduced Luxol fast blue staining in this area indicates demyelination. Note the darker blue staining (indicating intact myelin) of the U fibers adjacent to the cortex (C) at the bottom of a sulcus. V, lateral ventricle.fig 3. Trichrome staining shows excessively thick collagen layers (green) in the walls of small veins and venules (arrows) in the area of demyelination, where these thick-walled veins were most numerous. They are usually more numerous near the angle of the lateral ventricle.fig 4. TUNEL staining in the area of demyelination shows positive cells (brown stain) in the wall of a blood vessel (arrowheads) and in the brain parenchyma (arrow). Inset A, The brown, TUNEL-stained cell in the parenchyma appears to be apoptotic histologically in that its blue-stained nucleus is condensed and split into two segments. Inset B, Another TUNEL-stained cell in the lesional white matter with nuclear fragmentation.fig 5. A moderate number of amyloid plaques are shown in the area of cortex that was counted. Inset, Double stained for β-amyloid (black) and interleukin-1 (red) (the latter stains activated microglia and macrophages)